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m0491s criterion pre cast gradient sds page gel  (Bio-Rad)


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    Bio-Rad m0491s criterion pre cast gradient sds page gel
    M0491s Criterion Pre Cast Gradient Sds Page Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 768 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m0491s+criterion+pre+cast+gradient+sds+page+gel/4%E2%80%9320%25+Criterion+TGX+Precast+Midi+Protein+Gel/pm35263593-224-81-88
    Average 96 stars, based on 768 article reviews
    m0491s criterion pre cast gradient sds page gel - by Bioz Stars, 2026-09
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    Bicinchoninic Acid Protein Assay:

    Article Title: Defining the mammalian coactivation of hepatic 12-h clock and lipid metabolism.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SRC-3 Cell Signaling Technology Cat# 2126, RRID: AB_823642 Rabbit polyclonal anti-XBP1s Biolegend Cat# 619501, RRID: AB_315907 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat# 11815S, RRID: AB_2616025 Rabbit polyclonal anti-ATF6-a Santa Cruz Biotechnology Cat# sc-22799, RRID: AB_2242950 Rabbit polyclonal anti-XBP1 Santa Cruz Biotechnology Cat# sc-7908, RRID: AB_2107620 Rabbit polyclonal anti-BMAL1 Abcam Cat# ab3350, RRID: AB_303729 Rabbit anti-beta-Actin Monoclonal Antibody, HRP Conjugated, Clone 13E5 Cell Signaling Technology Cat# 5125, RRID: AB_1903890 Bacterial and virus strains Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A XBP1s-pHAGE A gift from Dr. Xi Chen at BCM N/A Chemicals, peptides, and recombinant proteins Tunicamycin EMD Millipore Cat# 654380 Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A Hygromycin B Thermo Fisher Scientific Cat# 10687010 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778030 HEPES Thermo Fisher Scientific Cat# 15630080 SuperScript VILOTM Master Mix Thermo Fisher Scientific Cat# 11755050 Micrococcal Nuclease New England Biolabs Cat# M0247S Sodium Pyruvate Thermo Fisher Scientific Cat# 11360070 PEG-it Virus Precipitation Solution System Biosciences Cat# LV810A-1 RNase A, DNase and protease-free Thermo Fisher Scientific Cat# EN0531 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440047 Power SYBR Green Master Mix Thermo Fisher Scientific Cat# 4367660 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat# 26400036 Fetal Bovine Serum Thermo Fisher Scientific Cat# 10437028 D-glucose Sigma Cat# G8270 Sodium Acetate Sigma Cat# S2889 2-Mercaptoethanol Sigma Cat# M3148 DMSO Sigma Cat# D8418 NP-40 Sigma Cat# N3516 Sodium chloride Sigma Cat# S7653 Tris/glycine/SDS buffer Biorad Cat# 161-0772 TBST Cell Signaling Technology Cat# 9997 10% SDS Gibco Cat# 15553-035 Triton X-100 Promega Cat# H5142 Tween 20 Fisher Scientific Cat# BP337-100 BSA Sigma Cat# A9647 EDTA Cell Signaling Technology Cat# 7011S Nonfat dry milk Biorad Cat# 170-6404 BCA Protein Assay Reagent A Thermo Fisher Scientific Cat# 23223 (Continued on next page) Cell Reports 38, 110491, March 8, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER BCA Protein Assay Reagent B Thermo Fisher Scientific Cat# 23224 iBlot Gel transfer stacks Nitrocellulose Life Technologies Cat# IB301001 Sodium deoxycholate Sigma Cat# D6750 Mouse Genomic DNA Promega Cat# G3091 Ponceau S solution Sigma Cat# P7170 VivoGloTM Luciferin, In Vivo Grade Promega Cat# P1041 Restore western blot stripping buffer Thermo Fisher Scientific Cat# 21063 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15070063 Pellet Paint Co-Precipitant EMD Millipore Cat# 69049 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat# M0491S Criterion pre-cast gradient SDS-PAGE gel (4-20%) Bio-Rad Cat# 5671094 TaKaRa Ex Taq DNA Polymerase Clontech Cat# RR001A T4 DNA Ligase New England Biolabs Cat# M0202 Antarctic Phosphatase New England Biolabs Cat# M0289S Ethanol 200 proof Decon Labs Cat# 2701 Isopropanol Sigma Cat# I9516 cOmpleteTM Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOPTM Roche Cat# 4906845001 DMEM, high glucose Thermo Fisher Scientific Cat# 11965092 DMEM, no glucose Thermo Fisher Scientific Cat# 11966025 Proteinase K Thermo Fisher Scientific Cat# AM2544 0.45mM syringe filter Thermo Fisher Scientific Cat# 09-740-106 Critical commercial assays SimpleChIP Plus Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9005S PureLink RNA mini kit Life Technologies Cat# 12183025 Dual-luciferase Reporter assay system Promega Cat# E1960 Insulin ELISA kit EMD Millipore Cat# EZRMI-13K One touch ultra glucose meter and strips LifeScan, Inc N/A Deposited data GSE175598 GEO public repository GSE175598 Study ST002079/PR001319 NMDR public repository datatrack_id:3065 study_id:ST002079; http://dev.metabolomicsworkbench. org:22222/data/DRCCMetadata.php? .. Mode=Study&StudyID=ST002079& Access=DoqF5000 PASS01737 PeptideAtlas (ProteomeXchange consortium member) PASS01737; http://www.peptideatlas.org/ PASS/PASS01737 Experimental Models: Cell Lines Immortalized mouse embryonic fibroblasts (Zhu et al., 2017) N/A Eif2ak3-dluc or Eif2ak3-dluc with mutated XBP1s binding site stably expressing MEFs (Zhu et al., 2017) N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J male mice The Jackson Laboratory JAX:000664 Mouse: SRC-3 knockout male mice (Xu et al., 2000) N/A Mouse: Xbp1flx/flx male mice A gift from Dr. Xi Chen at BCM (Lee et al., 2008) N/A Mouse: Xbp1flx/flx;AlbCre male mice (Meng et al., 2020) N/A (Continued on next page) e2 Cell Reports 38, 110491, March 8, 2022

    In Vivo:

    Article Title: Defining the mammalian coactivation of hepatic 12-h clock and lipid metabolism.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SRC-3 Cell Signaling Technology Cat# 2126, RRID: AB_823642 Rabbit polyclonal anti-XBP1s Biolegend Cat# 619501, RRID: AB_315907 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat# 11815S, RRID: AB_2616025 Rabbit polyclonal anti-ATF6-a Santa Cruz Biotechnology Cat# sc-22799, RRID: AB_2242950 Rabbit polyclonal anti-XBP1 Santa Cruz Biotechnology Cat# sc-7908, RRID: AB_2107620 Rabbit polyclonal anti-BMAL1 Abcam Cat# ab3350, RRID: AB_303729 Rabbit anti-beta-Actin Monoclonal Antibody, HRP Conjugated, Clone 13E5 Cell Signaling Technology Cat# 5125, RRID: AB_1903890 Bacterial and virus strains Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A XBP1s-pHAGE A gift from Dr. Xi Chen at BCM N/A Chemicals, peptides, and recombinant proteins Tunicamycin EMD Millipore Cat# 654380 Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A Hygromycin B Thermo Fisher Scientific Cat# 10687010 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778030 HEPES Thermo Fisher Scientific Cat# 15630080 SuperScript VILOTM Master Mix Thermo Fisher Scientific Cat# 11755050 Micrococcal Nuclease New England Biolabs Cat# M0247S Sodium Pyruvate Thermo Fisher Scientific Cat# 11360070 PEG-it Virus Precipitation Solution System Biosciences Cat# LV810A-1 RNase A, DNase and protease-free Thermo Fisher Scientific Cat# EN0531 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440047 Power SYBR Green Master Mix Thermo Fisher Scientific Cat# 4367660 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat# 26400036 Fetal Bovine Serum Thermo Fisher Scientific Cat# 10437028 D-glucose Sigma Cat# G8270 Sodium Acetate Sigma Cat# S2889 2-Mercaptoethanol Sigma Cat# M3148 DMSO Sigma Cat# D8418 NP-40 Sigma Cat# N3516 Sodium chloride Sigma Cat# S7653 Tris/glycine/SDS buffer Biorad Cat# 161-0772 TBST Cell Signaling Technology Cat# 9997 10% SDS Gibco Cat# 15553-035 Triton X-100 Promega Cat# H5142 Tween 20 Fisher Scientific Cat# BP337-100 BSA Sigma Cat# A9647 EDTA Cell Signaling Technology Cat# 7011S Nonfat dry milk Biorad Cat# 170-6404 BCA Protein Assay Reagent A Thermo Fisher Scientific Cat# 23223 (Continued on next page) Cell Reports 38, 110491, March 8, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER BCA Protein Assay Reagent B Thermo Fisher Scientific Cat# 23224 iBlot Gel transfer stacks Nitrocellulose Life Technologies Cat# IB301001 Sodium deoxycholate Sigma Cat# D6750 Mouse Genomic DNA Promega Cat# G3091 Ponceau S solution Sigma Cat# P7170 VivoGloTM Luciferin, In Vivo Grade Promega Cat# P1041 Restore western blot stripping buffer Thermo Fisher Scientific Cat# 21063 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15070063 Pellet Paint Co-Precipitant EMD Millipore Cat# 69049 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat# M0491S Criterion pre-cast gradient SDS-PAGE gel (4-20%) Bio-Rad Cat# 5671094 TaKaRa Ex Taq DNA Polymerase Clontech Cat# RR001A T4 DNA Ligase New England Biolabs Cat# M0202 Antarctic Phosphatase New England Biolabs Cat# M0289S Ethanol 200 proof Decon Labs Cat# 2701 Isopropanol Sigma Cat# I9516 cOmpleteTM Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOPTM Roche Cat# 4906845001 DMEM, high glucose Thermo Fisher Scientific Cat# 11965092 DMEM, no glucose Thermo Fisher Scientific Cat# 11966025 Proteinase K Thermo Fisher Scientific Cat# AM2544 0.45mM syringe filter Thermo Fisher Scientific Cat# 09-740-106 Critical commercial assays SimpleChIP Plus Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9005S PureLink RNA mini kit Life Technologies Cat# 12183025 Dual-luciferase Reporter assay system Promega Cat# E1960 Insulin ELISA kit EMD Millipore Cat# EZRMI-13K One touch ultra glucose meter and strips LifeScan, Inc N/A Deposited data GSE175598 GEO public repository GSE175598 Study ST002079/PR001319 NMDR public repository datatrack_id:3065 study_id:ST002079; http://dev.metabolomicsworkbench. org:22222/data/DRCCMetadata.php? .. Mode=Study&StudyID=ST002079& Access=DoqF5000 PASS01737 PeptideAtlas (ProteomeXchange consortium member) PASS01737; http://www.peptideatlas.org/ PASS/PASS01737 Experimental Models: Cell Lines Immortalized mouse embryonic fibroblasts (Zhu et al., 2017) N/A Eif2ak3-dluc or Eif2ak3-dluc with mutated XBP1s binding site stably expressing MEFs (Zhu et al., 2017) N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J male mice The Jackson Laboratory JAX:000664 Mouse: SRC-3 knockout male mice (Xu et al., 2000) N/A Mouse: Xbp1flx/flx male mice A gift from Dr. Xi Chen at BCM (Lee et al., 2008) N/A Mouse: Xbp1flx/flx;AlbCre male mice (Meng et al., 2020) N/A (Continued on next page) e2 Cell Reports 38, 110491, March 8, 2022

    Western Blot:

    Article Title: Defining the mammalian coactivation of hepatic 12-h clock and lipid metabolism.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SRC-3 Cell Signaling Technology Cat# 2126, RRID: AB_823642 Rabbit polyclonal anti-XBP1s Biolegend Cat# 619501, RRID: AB_315907 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat# 11815S, RRID: AB_2616025 Rabbit polyclonal anti-ATF6-a Santa Cruz Biotechnology Cat# sc-22799, RRID: AB_2242950 Rabbit polyclonal anti-XBP1 Santa Cruz Biotechnology Cat# sc-7908, RRID: AB_2107620 Rabbit polyclonal anti-BMAL1 Abcam Cat# ab3350, RRID: AB_303729 Rabbit anti-beta-Actin Monoclonal Antibody, HRP Conjugated, Clone 13E5 Cell Signaling Technology Cat# 5125, RRID: AB_1903890 Bacterial and virus strains Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A XBP1s-pHAGE A gift from Dr. Xi Chen at BCM N/A Chemicals, peptides, and recombinant proteins Tunicamycin EMD Millipore Cat# 654380 Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A Hygromycin B Thermo Fisher Scientific Cat# 10687010 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778030 HEPES Thermo Fisher Scientific Cat# 15630080 SuperScript VILOTM Master Mix Thermo Fisher Scientific Cat# 11755050 Micrococcal Nuclease New England Biolabs Cat# M0247S Sodium Pyruvate Thermo Fisher Scientific Cat# 11360070 PEG-it Virus Precipitation Solution System Biosciences Cat# LV810A-1 RNase A, DNase and protease-free Thermo Fisher Scientific Cat# EN0531 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440047 Power SYBR Green Master Mix Thermo Fisher Scientific Cat# 4367660 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat# 26400036 Fetal Bovine Serum Thermo Fisher Scientific Cat# 10437028 D-glucose Sigma Cat# G8270 Sodium Acetate Sigma Cat# S2889 2-Mercaptoethanol Sigma Cat# M3148 DMSO Sigma Cat# D8418 NP-40 Sigma Cat# N3516 Sodium chloride Sigma Cat# S7653 Tris/glycine/SDS buffer Biorad Cat# 161-0772 TBST Cell Signaling Technology Cat# 9997 10% SDS Gibco Cat# 15553-035 Triton X-100 Promega Cat# H5142 Tween 20 Fisher Scientific Cat# BP337-100 BSA Sigma Cat# A9647 EDTA Cell Signaling Technology Cat# 7011S Nonfat dry milk Biorad Cat# 170-6404 BCA Protein Assay Reagent A Thermo Fisher Scientific Cat# 23223 (Continued on next page) Cell Reports 38, 110491, March 8, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER BCA Protein Assay Reagent B Thermo Fisher Scientific Cat# 23224 iBlot Gel transfer stacks Nitrocellulose Life Technologies Cat# IB301001 Sodium deoxycholate Sigma Cat# D6750 Mouse Genomic DNA Promega Cat# G3091 Ponceau S solution Sigma Cat# P7170 VivoGloTM Luciferin, In Vivo Grade Promega Cat# P1041 Restore western blot stripping buffer Thermo Fisher Scientific Cat# 21063 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15070063 Pellet Paint Co-Precipitant EMD Millipore Cat# 69049 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat# M0491S Criterion pre-cast gradient SDS-PAGE gel (4-20%) Bio-Rad Cat# 5671094 TaKaRa Ex Taq DNA Polymerase Clontech Cat# RR001A T4 DNA Ligase New England Biolabs Cat# M0202 Antarctic Phosphatase New England Biolabs Cat# M0289S Ethanol 200 proof Decon Labs Cat# 2701 Isopropanol Sigma Cat# I9516 cOmpleteTM Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOPTM Roche Cat# 4906845001 DMEM, high glucose Thermo Fisher Scientific Cat# 11965092 DMEM, no glucose Thermo Fisher Scientific Cat# 11966025 Proteinase K Thermo Fisher Scientific Cat# AM2544 0.45mM syringe filter Thermo Fisher Scientific Cat# 09-740-106 Critical commercial assays SimpleChIP Plus Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9005S PureLink RNA mini kit Life Technologies Cat# 12183025 Dual-luciferase Reporter assay system Promega Cat# E1960 Insulin ELISA kit EMD Millipore Cat# EZRMI-13K One touch ultra glucose meter and strips LifeScan, Inc N/A Deposited data GSE175598 GEO public repository GSE175598 Study ST002079/PR001319 NMDR public repository datatrack_id:3065 study_id:ST002079; http://dev.metabolomicsworkbench. org:22222/data/DRCCMetadata.php? .. Mode=Study&StudyID=ST002079& Access=DoqF5000 PASS01737 PeptideAtlas (ProteomeXchange consortium member) PASS01737; http://www.peptideatlas.org/ PASS/PASS01737 Experimental Models: Cell Lines Immortalized mouse embryonic fibroblasts (Zhu et al., 2017) N/A Eif2ak3-dluc or Eif2ak3-dluc with mutated XBP1s binding site stably expressing MEFs (Zhu et al., 2017) N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J male mice The Jackson Laboratory JAX:000664 Mouse: SRC-3 knockout male mice (Xu et al., 2000) N/A Mouse: Xbp1flx/flx male mice A gift from Dr. Xi Chen at BCM (Lee et al., 2008) N/A Mouse: Xbp1flx/flx;AlbCre male mice (Meng et al., 2020) N/A (Continued on next page) e2 Cell Reports 38, 110491, March 8, 2022

    Stripping:

    Article Title: Defining the mammalian coactivation of hepatic 12-h clock and lipid metabolism.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SRC-3 Cell Signaling Technology Cat# 2126, RRID: AB_823642 Rabbit polyclonal anti-XBP1s Biolegend Cat# 619501, RRID: AB_315907 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat# 11815S, RRID: AB_2616025 Rabbit polyclonal anti-ATF6-a Santa Cruz Biotechnology Cat# sc-22799, RRID: AB_2242950 Rabbit polyclonal anti-XBP1 Santa Cruz Biotechnology Cat# sc-7908, RRID: AB_2107620 Rabbit polyclonal anti-BMAL1 Abcam Cat# ab3350, RRID: AB_303729 Rabbit anti-beta-Actin Monoclonal Antibody, HRP Conjugated, Clone 13E5 Cell Signaling Technology Cat# 5125, RRID: AB_1903890 Bacterial and virus strains Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A XBP1s-pHAGE A gift from Dr. Xi Chen at BCM N/A Chemicals, peptides, and recombinant proteins Tunicamycin EMD Millipore Cat# 654380 Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A Hygromycin B Thermo Fisher Scientific Cat# 10687010 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778030 HEPES Thermo Fisher Scientific Cat# 15630080 SuperScript VILOTM Master Mix Thermo Fisher Scientific Cat# 11755050 Micrococcal Nuclease New England Biolabs Cat# M0247S Sodium Pyruvate Thermo Fisher Scientific Cat# 11360070 PEG-it Virus Precipitation Solution System Biosciences Cat# LV810A-1 RNase A, DNase and protease-free Thermo Fisher Scientific Cat# EN0531 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440047 Power SYBR Green Master Mix Thermo Fisher Scientific Cat# 4367660 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat# 26400036 Fetal Bovine Serum Thermo Fisher Scientific Cat# 10437028 D-glucose Sigma Cat# G8270 Sodium Acetate Sigma Cat# S2889 2-Mercaptoethanol Sigma Cat# M3148 DMSO Sigma Cat# D8418 NP-40 Sigma Cat# N3516 Sodium chloride Sigma Cat# S7653 Tris/glycine/SDS buffer Biorad Cat# 161-0772 TBST Cell Signaling Technology Cat# 9997 10% SDS Gibco Cat# 15553-035 Triton X-100 Promega Cat# H5142 Tween 20 Fisher Scientific Cat# BP337-100 BSA Sigma Cat# A9647 EDTA Cell Signaling Technology Cat# 7011S Nonfat dry milk Biorad Cat# 170-6404 BCA Protein Assay Reagent A Thermo Fisher Scientific Cat# 23223 (Continued on next page) Cell Reports 38, 110491, March 8, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER BCA Protein Assay Reagent B Thermo Fisher Scientific Cat# 23224 iBlot Gel transfer stacks Nitrocellulose Life Technologies Cat# IB301001 Sodium deoxycholate Sigma Cat# D6750 Mouse Genomic DNA Promega Cat# G3091 Ponceau S solution Sigma Cat# P7170 VivoGloTM Luciferin, In Vivo Grade Promega Cat# P1041 Restore western blot stripping buffer Thermo Fisher Scientific Cat# 21063 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15070063 Pellet Paint Co-Precipitant EMD Millipore Cat# 69049 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat# M0491S Criterion pre-cast gradient SDS-PAGE gel (4-20%) Bio-Rad Cat# 5671094 TaKaRa Ex Taq DNA Polymerase Clontech Cat# RR001A T4 DNA Ligase New England Biolabs Cat# M0202 Antarctic Phosphatase New England Biolabs Cat# M0289S Ethanol 200 proof Decon Labs Cat# 2701 Isopropanol Sigma Cat# I9516 cOmpleteTM Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOPTM Roche Cat# 4906845001 DMEM, high glucose Thermo Fisher Scientific Cat# 11965092 DMEM, no glucose Thermo Fisher Scientific Cat# 11966025 Proteinase K Thermo Fisher Scientific Cat# AM2544 0.45mM syringe filter Thermo Fisher Scientific Cat# 09-740-106 Critical commercial assays SimpleChIP Plus Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9005S PureLink RNA mini kit Life Technologies Cat# 12183025 Dual-luciferase Reporter assay system Promega Cat# E1960 Insulin ELISA kit EMD Millipore Cat# EZRMI-13K One touch ultra glucose meter and strips LifeScan, Inc N/A Deposited data GSE175598 GEO public repository GSE175598 Study ST002079/PR001319 NMDR public repository datatrack_id:3065 study_id:ST002079; http://dev.metabolomicsworkbench. org:22222/data/DRCCMetadata.php? .. Mode=Study&StudyID=ST002079& Access=DoqF5000 PASS01737 PeptideAtlas (ProteomeXchange consortium member) PASS01737; http://www.peptideatlas.org/ PASS/PASS01737 Experimental Models: Cell Lines Immortalized mouse embryonic fibroblasts (Zhu et al., 2017) N/A Eif2ak3-dluc or Eif2ak3-dluc with mutated XBP1s binding site stably expressing MEFs (Zhu et al., 2017) N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J male mice The Jackson Laboratory JAX:000664 Mouse: SRC-3 knockout male mice (Xu et al., 2000) N/A Mouse: Xbp1flx/flx male mice A gift from Dr. Xi Chen at BCM (Lee et al., 2008) N/A Mouse: Xbp1flx/flx;AlbCre male mice (Meng et al., 2020) N/A (Continued on next page) e2 Cell Reports 38, 110491, March 8, 2022

    SDS Page:

    Article Title: Defining the mammalian coactivation of hepatic 12-h clock and lipid metabolism.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SRC-3 Cell Signaling Technology Cat# 2126, RRID: AB_823642 Rabbit polyclonal anti-XBP1s Biolegend Cat# 619501, RRID: AB_315907 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat# 11815S, RRID: AB_2616025 Rabbit polyclonal anti-ATF6-a Santa Cruz Biotechnology Cat# sc-22799, RRID: AB_2242950 Rabbit polyclonal anti-XBP1 Santa Cruz Biotechnology Cat# sc-7908, RRID: AB_2107620 Rabbit polyclonal anti-BMAL1 Abcam Cat# ab3350, RRID: AB_303729 Rabbit anti-beta-Actin Monoclonal Antibody, HRP Conjugated, Clone 13E5 Cell Signaling Technology Cat# 5125, RRID: AB_1903890 Bacterial and virus strains Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A XBP1s-pHAGE A gift from Dr. Xi Chen at BCM N/A Chemicals, peptides, and recombinant proteins Tunicamycin EMD Millipore Cat# 654380 Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A Hygromycin B Thermo Fisher Scientific Cat# 10687010 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778030 HEPES Thermo Fisher Scientific Cat# 15630080 SuperScript VILOTM Master Mix Thermo Fisher Scientific Cat# 11755050 Micrococcal Nuclease New England Biolabs Cat# M0247S Sodium Pyruvate Thermo Fisher Scientific Cat# 11360070 PEG-it Virus Precipitation Solution System Biosciences Cat# LV810A-1 RNase A, DNase and protease-free Thermo Fisher Scientific Cat# EN0531 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440047 Power SYBR Green Master Mix Thermo Fisher Scientific Cat# 4367660 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat# 26400036 Fetal Bovine Serum Thermo Fisher Scientific Cat# 10437028 D-glucose Sigma Cat# G8270 Sodium Acetate Sigma Cat# S2889 2-Mercaptoethanol Sigma Cat# M3148 DMSO Sigma Cat# D8418 NP-40 Sigma Cat# N3516 Sodium chloride Sigma Cat# S7653 Tris/glycine/SDS buffer Biorad Cat# 161-0772 TBST Cell Signaling Technology Cat# 9997 10% SDS Gibco Cat# 15553-035 Triton X-100 Promega Cat# H5142 Tween 20 Fisher Scientific Cat# BP337-100 BSA Sigma Cat# A9647 EDTA Cell Signaling Technology Cat# 7011S Nonfat dry milk Biorad Cat# 170-6404 BCA Protein Assay Reagent A Thermo Fisher Scientific Cat# 23223 (Continued on next page) Cell Reports 38, 110491, March 8, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER BCA Protein Assay Reagent B Thermo Fisher Scientific Cat# 23224 iBlot Gel transfer stacks Nitrocellulose Life Technologies Cat# IB301001 Sodium deoxycholate Sigma Cat# D6750 Mouse Genomic DNA Promega Cat# G3091 Ponceau S solution Sigma Cat# P7170 VivoGloTM Luciferin, In Vivo Grade Promega Cat# P1041 Restore western blot stripping buffer Thermo Fisher Scientific Cat# 21063 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15070063 Pellet Paint Co-Precipitant EMD Millipore Cat# 69049 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat# M0491S Criterion pre-cast gradient SDS-PAGE gel (4-20%) Bio-Rad Cat# 5671094 TaKaRa Ex Taq DNA Polymerase Clontech Cat# RR001A T4 DNA Ligase New England Biolabs Cat# M0202 Antarctic Phosphatase New England Biolabs Cat# M0289S Ethanol 200 proof Decon Labs Cat# 2701 Isopropanol Sigma Cat# I9516 cOmpleteTM Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOPTM Roche Cat# 4906845001 DMEM, high glucose Thermo Fisher Scientific Cat# 11965092 DMEM, no glucose Thermo Fisher Scientific Cat# 11966025 Proteinase K Thermo Fisher Scientific Cat# AM2544 0.45mM syringe filter Thermo Fisher Scientific Cat# 09-740-106 Critical commercial assays SimpleChIP Plus Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9005S PureLink RNA mini kit Life Technologies Cat# 12183025 Dual-luciferase Reporter assay system Promega Cat# E1960 Insulin ELISA kit EMD Millipore Cat# EZRMI-13K One touch ultra glucose meter and strips LifeScan, Inc N/A Deposited data GSE175598 GEO public repository GSE175598 Study ST002079/PR001319 NMDR public repository datatrack_id:3065 study_id:ST002079; http://dev.metabolomicsworkbench. org:22222/data/DRCCMetadata.php? .. Mode=Study&StudyID=ST002079& Access=DoqF5000 PASS01737 PeptideAtlas (ProteomeXchange consortium member) PASS01737; http://www.peptideatlas.org/ PASS/PASS01737 Experimental Models: Cell Lines Immortalized mouse embryonic fibroblasts (Zhu et al., 2017) N/A Eif2ak3-dluc or Eif2ak3-dluc with mutated XBP1s binding site stably expressing MEFs (Zhu et al., 2017) N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J male mice The Jackson Laboratory JAX:000664 Mouse: SRC-3 knockout male mice (Xu et al., 2000) N/A Mouse: Xbp1flx/flx male mice A gift from Dr. Xi Chen at BCM (Lee et al., 2008) N/A Mouse: Xbp1flx/flx;AlbCre male mice (Meng et al., 2020) N/A (Continued on next page) e2 Cell Reports 38, 110491, March 8, 2022

    Protease Inhibitor:

    Article Title: Defining the mammalian coactivation of hepatic 12-h clock and lipid metabolism.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SRC-3 Cell Signaling Technology Cat# 2126, RRID: AB_823642 Rabbit polyclonal anti-XBP1s Biolegend Cat# 619501, RRID: AB_315907 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat# 11815S, RRID: AB_2616025 Rabbit polyclonal anti-ATF6-a Santa Cruz Biotechnology Cat# sc-22799, RRID: AB_2242950 Rabbit polyclonal anti-XBP1 Santa Cruz Biotechnology Cat# sc-7908, RRID: AB_2107620 Rabbit polyclonal anti-BMAL1 Abcam Cat# ab3350, RRID: AB_303729 Rabbit anti-beta-Actin Monoclonal Antibody, HRP Conjugated, Clone 13E5 Cell Signaling Technology Cat# 5125, RRID: AB_1903890 Bacterial and virus strains Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A XBP1s-pHAGE A gift from Dr. Xi Chen at BCM N/A Chemicals, peptides, and recombinant proteins Tunicamycin EMD Millipore Cat# 654380 Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A Hygromycin B Thermo Fisher Scientific Cat# 10687010 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778030 HEPES Thermo Fisher Scientific Cat# 15630080 SuperScript VILOTM Master Mix Thermo Fisher Scientific Cat# 11755050 Micrococcal Nuclease New England Biolabs Cat# M0247S Sodium Pyruvate Thermo Fisher Scientific Cat# 11360070 PEG-it Virus Precipitation Solution System Biosciences Cat# LV810A-1 RNase A, DNase and protease-free Thermo Fisher Scientific Cat# EN0531 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440047 Power SYBR Green Master Mix Thermo Fisher Scientific Cat# 4367660 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat# 26400036 Fetal Bovine Serum Thermo Fisher Scientific Cat# 10437028 D-glucose Sigma Cat# G8270 Sodium Acetate Sigma Cat# S2889 2-Mercaptoethanol Sigma Cat# M3148 DMSO Sigma Cat# D8418 NP-40 Sigma Cat# N3516 Sodium chloride Sigma Cat# S7653 Tris/glycine/SDS buffer Biorad Cat# 161-0772 TBST Cell Signaling Technology Cat# 9997 10% SDS Gibco Cat# 15553-035 Triton X-100 Promega Cat# H5142 Tween 20 Fisher Scientific Cat# BP337-100 BSA Sigma Cat# A9647 EDTA Cell Signaling Technology Cat# 7011S Nonfat dry milk Biorad Cat# 170-6404 BCA Protein Assay Reagent A Thermo Fisher Scientific Cat# 23223 (Continued on next page) Cell Reports 38, 110491, March 8, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER BCA Protein Assay Reagent B Thermo Fisher Scientific Cat# 23224 iBlot Gel transfer stacks Nitrocellulose Life Technologies Cat# IB301001 Sodium deoxycholate Sigma Cat# D6750 Mouse Genomic DNA Promega Cat# G3091 Ponceau S solution Sigma Cat# P7170 VivoGloTM Luciferin, In Vivo Grade Promega Cat# P1041 Restore western blot stripping buffer Thermo Fisher Scientific Cat# 21063 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15070063 Pellet Paint Co-Precipitant EMD Millipore Cat# 69049 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat# M0491S Criterion pre-cast gradient SDS-PAGE gel (4-20%) Bio-Rad Cat# 5671094 TaKaRa Ex Taq DNA Polymerase Clontech Cat# RR001A T4 DNA Ligase New England Biolabs Cat# M0202 Antarctic Phosphatase New England Biolabs Cat# M0289S Ethanol 200 proof Decon Labs Cat# 2701 Isopropanol Sigma Cat# I9516 cOmpleteTM Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOPTM Roche Cat# 4906845001 DMEM, high glucose Thermo Fisher Scientific Cat# 11965092 DMEM, no glucose Thermo Fisher Scientific Cat# 11966025 Proteinase K Thermo Fisher Scientific Cat# AM2544 0.45mM syringe filter Thermo Fisher Scientific Cat# 09-740-106 Critical commercial assays SimpleChIP Plus Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9005S PureLink RNA mini kit Life Technologies Cat# 12183025 Dual-luciferase Reporter assay system Promega Cat# E1960 Insulin ELISA kit EMD Millipore Cat# EZRMI-13K One touch ultra glucose meter and strips LifeScan, Inc N/A Deposited data GSE175598 GEO public repository GSE175598 Study ST002079/PR001319 NMDR public repository datatrack_id:3065 study_id:ST002079; http://dev.metabolomicsworkbench. org:22222/data/DRCCMetadata.php? .. Mode=Study&StudyID=ST002079& Access=DoqF5000 PASS01737 PeptideAtlas (ProteomeXchange consortium member) PASS01737; http://www.peptideatlas.org/ PASS/PASS01737 Experimental Models: Cell Lines Immortalized mouse embryonic fibroblasts (Zhu et al., 2017) N/A Eif2ak3-dluc or Eif2ak3-dluc with mutated XBP1s binding site stably expressing MEFs (Zhu et al., 2017) N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J male mice The Jackson Laboratory JAX:000664 Mouse: SRC-3 knockout male mice (Xu et al., 2000) N/A Mouse: Xbp1flx/flx male mice A gift from Dr. Xi Chen at BCM (Lee et al., 2008) N/A Mouse: Xbp1flx/flx;AlbCre male mice (Meng et al., 2020) N/A (Continued on next page) e2 Cell Reports 38, 110491, March 8, 2022

    Chromatin Immunoprecipitation:

    Article Title: Defining the mammalian coactivation of hepatic 12-h clock and lipid metabolism.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SRC-3 Cell Signaling Technology Cat# 2126, RRID: AB_823642 Rabbit polyclonal anti-XBP1s Biolegend Cat# 619501, RRID: AB_315907 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat# 11815S, RRID: AB_2616025 Rabbit polyclonal anti-ATF6-a Santa Cruz Biotechnology Cat# sc-22799, RRID: AB_2242950 Rabbit polyclonal anti-XBP1 Santa Cruz Biotechnology Cat# sc-7908, RRID: AB_2107620 Rabbit polyclonal anti-BMAL1 Abcam Cat# ab3350, RRID: AB_303729 Rabbit anti-beta-Actin Monoclonal Antibody, HRP Conjugated, Clone 13E5 Cell Signaling Technology Cat# 5125, RRID: AB_1903890 Bacterial and virus strains Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A XBP1s-pHAGE A gift from Dr. Xi Chen at BCM N/A Chemicals, peptides, and recombinant proteins Tunicamycin EMD Millipore Cat# 654380 Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A Hygromycin B Thermo Fisher Scientific Cat# 10687010 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778030 HEPES Thermo Fisher Scientific Cat# 15630080 SuperScript VILOTM Master Mix Thermo Fisher Scientific Cat# 11755050 Micrococcal Nuclease New England Biolabs Cat# M0247S Sodium Pyruvate Thermo Fisher Scientific Cat# 11360070 PEG-it Virus Precipitation Solution System Biosciences Cat# LV810A-1 RNase A, DNase and protease-free Thermo Fisher Scientific Cat# EN0531 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440047 Power SYBR Green Master Mix Thermo Fisher Scientific Cat# 4367660 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat# 26400036 Fetal Bovine Serum Thermo Fisher Scientific Cat# 10437028 D-glucose Sigma Cat# G8270 Sodium Acetate Sigma Cat# S2889 2-Mercaptoethanol Sigma Cat# M3148 DMSO Sigma Cat# D8418 NP-40 Sigma Cat# N3516 Sodium chloride Sigma Cat# S7653 Tris/glycine/SDS buffer Biorad Cat# 161-0772 TBST Cell Signaling Technology Cat# 9997 10% SDS Gibco Cat# 15553-035 Triton X-100 Promega Cat# H5142 Tween 20 Fisher Scientific Cat# BP337-100 BSA Sigma Cat# A9647 EDTA Cell Signaling Technology Cat# 7011S Nonfat dry milk Biorad Cat# 170-6404 BCA Protein Assay Reagent A Thermo Fisher Scientific Cat# 23223 (Continued on next page) Cell Reports 38, 110491, March 8, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER BCA Protein Assay Reagent B Thermo Fisher Scientific Cat# 23224 iBlot Gel transfer stacks Nitrocellulose Life Technologies Cat# IB301001 Sodium deoxycholate Sigma Cat# D6750 Mouse Genomic DNA Promega Cat# G3091 Ponceau S solution Sigma Cat# P7170 VivoGloTM Luciferin, In Vivo Grade Promega Cat# P1041 Restore western blot stripping buffer Thermo Fisher Scientific Cat# 21063 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15070063 Pellet Paint Co-Precipitant EMD Millipore Cat# 69049 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat# M0491S Criterion pre-cast gradient SDS-PAGE gel (4-20%) Bio-Rad Cat# 5671094 TaKaRa Ex Taq DNA Polymerase Clontech Cat# RR001A T4 DNA Ligase New England Biolabs Cat# M0202 Antarctic Phosphatase New England Biolabs Cat# M0289S Ethanol 200 proof Decon Labs Cat# 2701 Isopropanol Sigma Cat# I9516 cOmpleteTM Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOPTM Roche Cat# 4906845001 DMEM, high glucose Thermo Fisher Scientific Cat# 11965092 DMEM, no glucose Thermo Fisher Scientific Cat# 11966025 Proteinase K Thermo Fisher Scientific Cat# AM2544 0.45mM syringe filter Thermo Fisher Scientific Cat# 09-740-106 Critical commercial assays SimpleChIP Plus Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9005S PureLink RNA mini kit Life Technologies Cat# 12183025 Dual-luciferase Reporter assay system Promega Cat# E1960 Insulin ELISA kit EMD Millipore Cat# EZRMI-13K One touch ultra glucose meter and strips LifeScan, Inc N/A Deposited data GSE175598 GEO public repository GSE175598 Study ST002079/PR001319 NMDR public repository datatrack_id:3065 study_id:ST002079; http://dev.metabolomicsworkbench. org:22222/data/DRCCMetadata.php? .. Mode=Study&StudyID=ST002079& Access=DoqF5000 PASS01737 PeptideAtlas (ProteomeXchange consortium member) PASS01737; http://www.peptideatlas.org/ PASS/PASS01737 Experimental Models: Cell Lines Immortalized mouse embryonic fibroblasts (Zhu et al., 2017) N/A Eif2ak3-dluc or Eif2ak3-dluc with mutated XBP1s binding site stably expressing MEFs (Zhu et al., 2017) N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J male mice The Jackson Laboratory JAX:000664 Mouse: SRC-3 knockout male mice (Xu et al., 2000) N/A Mouse: Xbp1flx/flx male mice A gift from Dr. Xi Chen at BCM (Lee et al., 2008) N/A Mouse: Xbp1flx/flx;AlbCre male mice (Meng et al., 2020) N/A (Continued on next page) e2 Cell Reports 38, 110491, March 8, 2022

    Reporter Assay:

    Article Title: Defining the mammalian coactivation of hepatic 12-h clock and lipid metabolism.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SRC-3 Cell Signaling Technology Cat# 2126, RRID: AB_823642 Rabbit polyclonal anti-XBP1s Biolegend Cat# 619501, RRID: AB_315907 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat# 11815S, RRID: AB_2616025 Rabbit polyclonal anti-ATF6-a Santa Cruz Biotechnology Cat# sc-22799, RRID: AB_2242950 Rabbit polyclonal anti-XBP1 Santa Cruz Biotechnology Cat# sc-7908, RRID: AB_2107620 Rabbit polyclonal anti-BMAL1 Abcam Cat# ab3350, RRID: AB_303729 Rabbit anti-beta-Actin Monoclonal Antibody, HRP Conjugated, Clone 13E5 Cell Signaling Technology Cat# 5125, RRID: AB_1903890 Bacterial and virus strains Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A XBP1s-pHAGE A gift from Dr. Xi Chen at BCM N/A Chemicals, peptides, and recombinant proteins Tunicamycin EMD Millipore Cat# 654380 Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A Hygromycin B Thermo Fisher Scientific Cat# 10687010 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778030 HEPES Thermo Fisher Scientific Cat# 15630080 SuperScript VILOTM Master Mix Thermo Fisher Scientific Cat# 11755050 Micrococcal Nuclease New England Biolabs Cat# M0247S Sodium Pyruvate Thermo Fisher Scientific Cat# 11360070 PEG-it Virus Precipitation Solution System Biosciences Cat# LV810A-1 RNase A, DNase and protease-free Thermo Fisher Scientific Cat# EN0531 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440047 Power SYBR Green Master Mix Thermo Fisher Scientific Cat# 4367660 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat# 26400036 Fetal Bovine Serum Thermo Fisher Scientific Cat# 10437028 D-glucose Sigma Cat# G8270 Sodium Acetate Sigma Cat# S2889 2-Mercaptoethanol Sigma Cat# M3148 DMSO Sigma Cat# D8418 NP-40 Sigma Cat# N3516 Sodium chloride Sigma Cat# S7653 Tris/glycine/SDS buffer Biorad Cat# 161-0772 TBST Cell Signaling Technology Cat# 9997 10% SDS Gibco Cat# 15553-035 Triton X-100 Promega Cat# H5142 Tween 20 Fisher Scientific Cat# BP337-100 BSA Sigma Cat# A9647 EDTA Cell Signaling Technology Cat# 7011S Nonfat dry milk Biorad Cat# 170-6404 BCA Protein Assay Reagent A Thermo Fisher Scientific Cat# 23223 (Continued on next page) Cell Reports 38, 110491, March 8, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER BCA Protein Assay Reagent B Thermo Fisher Scientific Cat# 23224 iBlot Gel transfer stacks Nitrocellulose Life Technologies Cat# IB301001 Sodium deoxycholate Sigma Cat# D6750 Mouse Genomic DNA Promega Cat# G3091 Ponceau S solution Sigma Cat# P7170 VivoGloTM Luciferin, In Vivo Grade Promega Cat# P1041 Restore western blot stripping buffer Thermo Fisher Scientific Cat# 21063 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15070063 Pellet Paint Co-Precipitant EMD Millipore Cat# 69049 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat# M0491S Criterion pre-cast gradient SDS-PAGE gel (4-20%) Bio-Rad Cat# 5671094 TaKaRa Ex Taq DNA Polymerase Clontech Cat# RR001A T4 DNA Ligase New England Biolabs Cat# M0202 Antarctic Phosphatase New England Biolabs Cat# M0289S Ethanol 200 proof Decon Labs Cat# 2701 Isopropanol Sigma Cat# I9516 cOmpleteTM Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOPTM Roche Cat# 4906845001 DMEM, high glucose Thermo Fisher Scientific Cat# 11965092 DMEM, no glucose Thermo Fisher Scientific Cat# 11966025 Proteinase K Thermo Fisher Scientific Cat# AM2544 0.45mM syringe filter Thermo Fisher Scientific Cat# 09-740-106 Critical commercial assays SimpleChIP Plus Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9005S PureLink RNA mini kit Life Technologies Cat# 12183025 Dual-luciferase Reporter assay system Promega Cat# E1960 Insulin ELISA kit EMD Millipore Cat# EZRMI-13K One touch ultra glucose meter and strips LifeScan, Inc N/A Deposited data GSE175598 GEO public repository GSE175598 Study ST002079/PR001319 NMDR public repository datatrack_id:3065 study_id:ST002079; http://dev.metabolomicsworkbench. org:22222/data/DRCCMetadata.php? .. Mode=Study&StudyID=ST002079& Access=DoqF5000 PASS01737 PeptideAtlas (ProteomeXchange consortium member) PASS01737; http://www.peptideatlas.org/ PASS/PASS01737 Experimental Models: Cell Lines Immortalized mouse embryonic fibroblasts (Zhu et al., 2017) N/A Eif2ak3-dluc or Eif2ak3-dluc with mutated XBP1s binding site stably expressing MEFs (Zhu et al., 2017) N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J male mice The Jackson Laboratory JAX:000664 Mouse: SRC-3 knockout male mice (Xu et al., 2000) N/A Mouse: Xbp1flx/flx male mice A gift from Dr. Xi Chen at BCM (Lee et al., 2008) N/A Mouse: Xbp1flx/flx;AlbCre male mice (Meng et al., 2020) N/A (Continued on next page) e2 Cell Reports 38, 110491, March 8, 2022

    Enzyme-linked Immunosorbent Assay:

    Article Title: Defining the mammalian coactivation of hepatic 12-h clock and lipid metabolism.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-SRC-3 Cell Signaling Technology Cat# 2126, RRID: AB_823642 Rabbit polyclonal anti-XBP1s Biolegend Cat# 619501, RRID: AB_315907 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat# 11815S, RRID: AB_2616025 Rabbit polyclonal anti-ATF6-a Santa Cruz Biotechnology Cat# sc-22799, RRID: AB_2242950 Rabbit polyclonal anti-XBP1 Santa Cruz Biotechnology Cat# sc-7908, RRID: AB_2107620 Rabbit polyclonal anti-BMAL1 Abcam Cat# ab3350, RRID: AB_303729 Rabbit anti-beta-Actin Monoclonal Antibody, HRP Conjugated, Clone 13E5 Cell Signaling Technology Cat# 5125, RRID: AB_1903890 Bacterial and virus strains Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A XBP1s-pHAGE A gift from Dr. Xi Chen at BCM N/A Chemicals, peptides, and recombinant proteins Tunicamycin EMD Millipore Cat# 654380 Eif2ak3 promoter-pGreenfire-mCMVdscGFP-EF1-Puro lentivirus (Zhu et al., 2017) N/A Hygromycin B Thermo Fisher Scientific Cat# 10687010 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat# 11668027 Lipofectamine RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778030 HEPES Thermo Fisher Scientific Cat# 15630080 SuperScript VILOTM Master Mix Thermo Fisher Scientific Cat# 11755050 Micrococcal Nuclease New England Biolabs Cat# M0247S Sodium Pyruvate Thermo Fisher Scientific Cat# 11360070 PEG-it Virus Precipitation Solution System Biosciences Cat# LV810A-1 RNase A, DNase and protease-free Thermo Fisher Scientific Cat# EN0531 TaqMan Universal Master Mix II, no UNG Thermo Fisher Scientific Cat# 4440047 Power SYBR Green Master Mix Thermo Fisher Scientific Cat# 4367660 Dialyzed Fetal Bovine Serum Thermo Fisher Scientific Cat# 26400036 Fetal Bovine Serum Thermo Fisher Scientific Cat# 10437028 D-glucose Sigma Cat# G8270 Sodium Acetate Sigma Cat# S2889 2-Mercaptoethanol Sigma Cat# M3148 DMSO Sigma Cat# D8418 NP-40 Sigma Cat# N3516 Sodium chloride Sigma Cat# S7653 Tris/glycine/SDS buffer Biorad Cat# 161-0772 TBST Cell Signaling Technology Cat# 9997 10% SDS Gibco Cat# 15553-035 Triton X-100 Promega Cat# H5142 Tween 20 Fisher Scientific Cat# BP337-100 BSA Sigma Cat# A9647 EDTA Cell Signaling Technology Cat# 7011S Nonfat dry milk Biorad Cat# 170-6404 BCA Protein Assay Reagent A Thermo Fisher Scientific Cat# 23223 (Continued on next page) Cell Reports 38, 110491, March 8, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER BCA Protein Assay Reagent B Thermo Fisher Scientific Cat# 23224 iBlot Gel transfer stacks Nitrocellulose Life Technologies Cat# IB301001 Sodium deoxycholate Sigma Cat# D6750 Mouse Genomic DNA Promega Cat# G3091 Ponceau S solution Sigma Cat# P7170 VivoGloTM Luciferin, In Vivo Grade Promega Cat# P1041 Restore western blot stripping buffer Thermo Fisher Scientific Cat# 21063 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15070063 Pellet Paint Co-Precipitant EMD Millipore Cat# 69049 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat# M0491S Criterion pre-cast gradient SDS-PAGE gel (4-20%) Bio-Rad Cat# 5671094 TaKaRa Ex Taq DNA Polymerase Clontech Cat# RR001A T4 DNA Ligase New England Biolabs Cat# M0202 Antarctic Phosphatase New England Biolabs Cat# M0289S Ethanol 200 proof Decon Labs Cat# 2701 Isopropanol Sigma Cat# I9516 cOmpleteTM Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOPTM Roche Cat# 4906845001 DMEM, high glucose Thermo Fisher Scientific Cat# 11965092 DMEM, no glucose Thermo Fisher Scientific Cat# 11966025 Proteinase K Thermo Fisher Scientific Cat# AM2544 0.45mM syringe filter Thermo Fisher Scientific Cat# 09-740-106 Critical commercial assays SimpleChIP Plus Enzymatic Chromatin IP Kit Cell Signaling Technology Cat# 9005S PureLink RNA mini kit Life Technologies Cat# 12183025 Dual-luciferase Reporter assay system Promega Cat# E1960 Insulin ELISA kit EMD Millipore Cat# EZRMI-13K One touch ultra glucose meter and strips LifeScan, Inc N/A Deposited data GSE175598 GEO public repository GSE175598 Study ST002079/PR001319 NMDR public repository datatrack_id:3065 study_id:ST002079; http://dev.metabolomicsworkbench. org:22222/data/DRCCMetadata.php? .. Mode=Study&StudyID=ST002079& Access=DoqF5000 PASS01737 PeptideAtlas (ProteomeXchange consortium member) PASS01737; http://www.peptideatlas.org/ PASS/PASS01737 Experimental Models: Cell Lines Immortalized mouse embryonic fibroblasts (Zhu et al., 2017) N/A Eif2ak3-dluc or Eif2ak3-dluc with mutated XBP1s binding site stably expressing MEFs (Zhu et al., 2017) N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J male mice The Jackson Laboratory JAX:000664 Mouse: SRC-3 knockout male mice (Xu et al., 2000) N/A Mouse: Xbp1flx/flx male mice A gift from Dr. Xi Chen at BCM (Lee et al., 2008) N/A Mouse: Xbp1flx/flx;AlbCre male mice (Meng et al., 2020) N/A (Continued on next page) e2 Cell Reports 38, 110491, March 8, 2022



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